View source for LCMS:Glycyrrhizae Radix

{{LCMS/Header}}
{{CrudeDrug/TIC
|TICFile=Glycyrrhizae LC-MS
}}

{{CrudeDrug/LCMS/Pos
|PInstrument =Shimadzu LC-IT-TOF MS ESI
|PColumn     =Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron)
|PTemperature=40
|PSolventA   =5 mM Ammonium acetate solution
|PSolventB   =acetonitrile
|PGradient   =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
|PVoltage    =4.5
|PCapillary  =200
|PGas        =1.5
}}

{{CrudeDrug/LCMS/Neg
|NInstrument =Shimadzu LC-IT-TOF MS ESI
|NColumn     =Waters Atlantis T3 (2.1 mm x 150 mm)
|NTemperature=40
|NSolventA   =5 mM Ammonium acetate solution
|NSolventB   =acetonitrile
|NGradient   =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
|NVoltage    =-3.5
|NCapillary  =200
|NGas        =1.5
}}

== 2D-Mass chromatogram (Condition 1) ==

[[Image:Glycyrrhizae_Radix_LC1.jpg]]

{{CrudeDrug/LCMS/Neg
|NInstrument =Shimadzu LC-IT-TOF MS ESI
|NColumn     =Waters Atlantis T3 (2.1 mm x 150 mm)
|NTemperature=40
|NSolventA   =5 mM Ammonium acetate solution
|NSolventB   =acetonitrile
|NGradient   =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
|NVoltage    =-3.5
|NCapillary  =200
|NGas        =1.5
}}

{| class="wikitable"
! Retention Time (min) !! Compounds !!Molecular formula !!(M-H)- ions
|-
! 12.60
| [[Liquiritin]] || C<sub>21</sub>H<sub>22</sub>O<sub>9</sub> || 417.1191
|-
! 12.85
| [[Liquiritin apioside]] || C<sub>26</sub>H<sub>22</sub>O<sub>9</sub> || 549.1614
|-
! 13.41
| [[Licoricesaponin G2]] || C<sub>42</sub>H<sub>62</sub>O<sub>17</sub> || 837.3914
|-
! 14.10
| [[Glycyrrhizin]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
|-
! 14.26
| [[Licoricesaponin A3]] || C<sub>48</sub>H<sub>72</sub>O<sub>21</sub> || 983.4493
|-
! 14.47
| [[Licoricesaponin H2]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
|-
! 14.92
| [[Licoricesaponin E2]] || C<sub>42</sub>H<sub>60</sub>O<sub>16</sub> || 819.3809
|-
! 16.12
| [[Licorice glycoside E]] || C<sub>35</sub>H<sub>35</sub>NO<sub>14</sub> || 692.1985
|}

{{JCampViewer|Glycyrrhizae_std_T3.JDX.gz}}

== 2D-Mass chromatogram (Condition 2) ==

[[Image:Glycyrrhizae_Radix_LC2.jpg]]

{{CrudeDrug/LCMS/Neg
|NInstrument =Shimadzu LC-IT-TOF MS ESI
|NColumn     =Waters Symmetry C18(2.1 mm x 150 mm)
|NTemperature=40
|NSolventA   =0.1% Formic acid solution
|NSolventB   =acetonitrile
|NGradient   =10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
|NVoltage    =-3.5
|NCapillary  =200
|NGas        =1.5
}}

{| class="wikitable"
! Retention Time (min) !! Compounds !!Molecular formula !!(M-H)- ions
|-
! 11.48
| [[Liquiritin]] || C<sub>21</sub>H<sub>22</sub>O<sub>9</sub> || 417.1191
|-
! 11.65
| [[Liquiritin apioside]] || C<sub>26</sub>H<sub>22</sub>O<sub>9</sub> || 549.1614
|-
! 14.84
| [[Licorice glycoside E]] || C<sub>35</sub>H<sub>35</sub>NO<sub>14</sub> || 692.1985
|-
! 16.28
| [[Licoricesaponin A3]] || C<sub>48</sub>H<sub>72</sub>O<sub>21</sub> || 983.4493
|-
! 18.59
| [[Licoricesaponin E2]] || C<sub>42</sub>H<sub>60</sub>O<sub>16</sub> || 819.3809
|-
! 18.71
| [[Licoricesaponin G2]] || C<sub>42</sub>H<sub>62</sub>O<sub>17</sub> || 837.3914
|-
! 19.21
| [[Glycyrrhizin]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
|-
! 20.19
| [[Licoricesaponin H2]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
|}