|
|
| Line 26: |
Line 26: |
| | |NGas =1.5 | | |NGas =1.5 |
| | }} | | }} |
| − |
| |
| − | == 2D-Mass chromatogram (Condition 1) ==
| |
| − | [[Image:Glycyrrhizae_Radix_LC1.jpg]]
| |
| − | {| class="wikitable" style="width:80%"
| |
| − | |-
| |
| − | | Instrument || Shimadzu LC-IT-TOF MS ESI (Negative ion mode)
| |
| − | |-
| |
| − | | Column || Waters Atlantis T3 (2.1 mm x 150 mm)
| |
| − | |-
| |
| − | | Column Temperature|| 40℃
| |
| − | |-
| |
| − | | Solvent A || 5 mM Ammonium acetate solution
| |
| − | |-
| |
| − | | Solvent B || acetonitrile
| |
| − | |-
| |
| − | | Gradient || 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
| |
| − | |-
| |
| − | | Source voltage || ? 3.5 kV
| |
| − | |-
| |
| − | | Capillary temperature || 200 °C
| |
| − | |-
| |
| − | | Nebulizer gas || 1.5 l/min
| |
| − | |}
| |
| − |
| |
| − | {| class="wikitable"
| |
| − | ! Retention Time (min) !! Compounds !!Molecular formula !!(M-H)- ions
| |
| − | |-
| |
| − | ! 12.60
| |
| − | | [[Liquiritin]] || C<sub>21</sub>H<sub>22</sub>O<sub>9</sub> || 417.1191
| |
| − | |-
| |
| − | ! 12.85
| |
| − | | [[Liquiritin apioside]] || C<sub>26</sub>H<sub>22</sub>O<sub>9</sub> || 549.1614
| |
| − | |-
| |
| − | ! 13.41
| |
| − | | [[Licoricesaponin G2]] || C<sub>42</sub>H<sub>62</sub>O<sub>17</sub> || 837.3914
| |
| − | |-
| |
| − | ! 14.10
| |
| − | | [[Glycyrrhizin]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
| |
| − | |-
| |
| − | ! 14.26
| |
| − | | [[Licoricesaponin A3]] || C<sub>48</sub>H<sub>72</sub>O<sub>21</sub> || 983.4493
| |
| − | |-
| |
| − | ! 14.47
| |
| − | | [[Licoricesaponin H2]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
| |
| − | |-
| |
| − | ! 14.92
| |
| − | | [[Licoricesaponin E2]] || C<sub>42</sub>H<sub>60</sub>O<sub>16</sub> || 819.3809
| |
| − | |-
| |
| − | ! 16.12
| |
| − | | [[Licorice glycoside E]] || C<sub>35</sub>H<sub>35</sub>NO<sub>14</sub> || 692.1985
| |
| − | |}
| |
| − |
| |
| − | {{JCampViewer|Glycyrrhizae_std_T3.JDX.gz}}
| |
| − |
| |
| − | == 2D-Mass chromatogram (Condition 2) ==
| |
| − |
| |
| − | [[Image:Glycyrrhizae_Radix_LC2.jpg]]
| |
| − | {| class="wikitable" style="width:80%"
| |
| − | |-
| |
| − | | Instrument || Shimadzu LC-IT-TOF MS ESI (Negative ion mode)
| |
| − | |-
| |
| − | | Column || Waters Symmetry C18(2.1 mm x 150 mm)
| |
| − | |-
| |
| − | | Column Temperature|| 40℃
| |
| − | |-
| |
| − | | Solvent A || 0.1% Formic acid solution
| |
| − | |-
| |
| − | | Solvent B || acetonitrile
| |
| − | |-
| |
| − | | Gradient || 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
| |
| − | |-
| |
| − | | Source voltage || ? 3.5 kV
| |
| − | |-
| |
| − | | Capillary temperature || 200 °C
| |
| − | |-
| |
| − | | Nebulizer gas || 1.5 l/min
| |
| − | |}
| |
| − |
| |
| − | {| class="wikitable"
| |
| − | ! Retention Time (min) !! Compounds !!Molecular formula !!(M-H)- ions
| |
| − | |-
| |
| − | ! 11.48
| |
| − | | [[Liquiritin]] || C<sub>21</sub>H<sub>22</sub>O<sub>9</sub> || 417.1191
| |
| − | |-
| |
| − | ! 11.65
| |
| − | | [[Liquiritin apioside]] || C<sub>26</sub>H<sub>22</sub>O<sub>9</sub> || 549.1614
| |
| − | |-
| |
| − | ! 14.84
| |
| − | | [[Licorice glycoside E]] || C<sub>35</sub>H<sub>35</sub>NO<sub>14</sub> || 692.1985
| |
| − | |-
| |
| − | ! 16.28
| |
| − | | [[Licoricesaponin A3]] || C<sub>48</sub>H<sub>72</sub>O<sub>21</sub> || 983.4493
| |
| − | |-
| |
| − | ! 18.59
| |
| − | | [[Licoricesaponin E2]] || C<sub>42</sub>H<sub>60</sub>O<sub>16</sub> || 819.3809
| |
| − | |-
| |
| − | ! 18.71
| |
| − | | [[Licoricesaponin G2]] || C<sub>42</sub>H<sub>62</sub>O<sub>17</sub> || 837.3914
| |
| − | |-
| |
| − | ! 19.21
| |
| − | | [[Glycyrrhizin]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
| |
| − | |-
| |
| − | ! 20.19
| |
| − | | [[Licoricesaponin H2]] || C<sub>42</sub>H<sub>62</sub>O<sub>16</sub> || 821.3965
| |
| − | |}
| |
