Sandbox000

2D-Mass chromatogram (Condition 1)

Instrument	Shimadzu LC-IT-TOF MS ESI (Negative ion mode)
Column	Waters Atlantis T3 (2.1 mm x 150 mm)
Column Temperature	40℃
Solvent A	5 mM Ammonium acetate solution
Solvent B	acetonitrile
Gradient	10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage	– 3.5 kV
Capillary temperature	200 °C
Nebulizer gas	1.5 l/min

Retention Time (min)	Compounds	Molecular formula	(M-H)- ions
12.60	Liquiritin	C21H22O9	417.1191 12.85
Liquiritin apioside	[[C26H22O9]]	549.1614 13.41 || Licoricesaponin G2
C42H62O17	[[837.3914 14.10]] || Glycyrrhizin || C42H62O16
821.3965 14.26	Licoricesaponin A3	C48H72O21	983.4493 14.47
Licoricesaponin H2	[[C42H62O16]]	821.3965 14.92 || Licoricesaponin E2
C42H60O16	[[819.3809 16.12]] || Licorice glycoside E || C35H35NO14
692.1985	[[]]