LCMS:Coptidis Rhizoma
From Metabolomics.JP
Contents |
TIC
[[Image:Coptidis LC-MS.jpg |thumb|700px|center|LC-MS TIC of extracts]]
Analytical conditions
| Instrument | Shimadzu LC-IT-TOF MS ESI (Positive ion mode) |
| Column | Waters Atlantis T3 (2.1 mm x 100 mm, 3 micron) |
| Column Temperature | 40℃ |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | 4.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
| Instrument | Shimadzu LC-IT-TOF MS ESI (Negative ion mode) |
| Column | Waters Atlantis T3 (2.1 mm x 150 mm) |
| Column Temperature | 40℃ |
| Solvent A | 5 mM Ammonium acetate solution |
| Solvent B | acetonitrile |
| Gradient | 10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min) |
| Source voltage | – 3.5 kV |
| Capillary temperature | 200 °C |
| Nebulizer gas | 1.5 l/min |
