LCMS:Glycyrrhizae Radix

Revision as of 17:56, 9 December 2009

Instrument	Shimadzu LC-IT-TOF MS ESI (Negative ion mode)
Column	Waters Atlantis T3 (2.1 mm x 150 mm)
Column Temperature	40℃
Solvent A	5 mM Ammonium acetate solution
Solvent B	acetonitrile
Gradient	10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage	– 3.5 kV
Capillary temperature	200 °C
Nebulizer gas	1.5 l/min

Retention Time (min)	Compounds	Molecular formula	(M-H)- ions
12.60	Liquiritin	C₂₁H₂₂O₉	417.1191
12.85	Liquiritin apioside	C₂₆H₂₂O₉	549.1614
13.41	Licoricesaponin G2	C₄₂H₆₂O₁₇	837.3914
14.10	Glycyrrhizin	C₄₂H₆₂O₁₆	821.3965
14.26	Licoricesaponin A3	C₄₈H₇₂O₂₁	983.4493
14.47	Licoricesaponin H2	C₄₂H₆₂O₁₆	821.3965
14.92	Licoricesaponin E2	C₄₂H₆₀O₁₆	819.3809
16.12	Licorice glycoside E	C₃₅H₃₅NO₁₄	692.1985

Instrument	Shimadzu LC-IT-TOF MS ESI (Negative ion mode)
Column	Waters Symmetry C18(2.1 mm x 150 mm)
Column Temperature	40℃
Solvent A	0.1% Formic acid solution
Solvent B	acetonitrile
Gradient	10% to 100% Solvent B (0-30 min), 100% Solvent B (30-40 min)
Source voltage	– 3.5 kV
Capillary temperature	200 °C
Nebulizer gas	1.5 l/min

Retention Time (min)	Compounds	Molecular formula	(M-H)- ions
11.48	Liquiritin	C₂₁H₂₂O₉	417.1191
11.65	Liquiritin apioside	C₂₆H₂₂O₉	549.1614
14.84	Licorice glycoside E	C₃₅H₃₅NO₁₄	692.1985
16.28	Licoricesaponin A3	C₄₈H₇₂O₂₁	983.4493
18.59	Licoricesaponin E2	C₄₂H₆₀O₁₆	819.3809
18.71	Licoricesaponin G2	C₄₂H₆₂O₁₇	837.3914
19.21	Glycyrrhizin	C₄₂H₆₂O₁₆	821.3965
20.19	Licoricesaponin H2	C₄₂H₆₂O₁₆	821.3965

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